Waters Waters nanoACQUITY Ultra Performance, Eksigent Technologies NanoLC-2D, and LC Packings' UltiMate Plus Capillary/Nano LC System are highlighted in a discussion of liquid chromatography (LC) products that concentrate on improving reproducibility and elimination of high-abundant proteins in the study of proteomics science. Bob Pfeifer, VP of life sciences R&D for Waters, says the industry has been working to transform the science from a discovery-based process to one that is more quantitative. Repeatability and robustness are requirements because proteomics scientists deal with complex samples made up of many components. Analytical tools are needed that are highly sensitive with sufficient resolution to allow measurement of a protein of interest. nanoACQUITY Ultra Performance LC (UPLC), which is a modified version of ACQUITY UPLC, runs with direct flow at 200nL/minute to 5muL/minute flow rates. Low rates are possible, and users can obtain greatly improved control over flow than with flow splitting. NanoACQUITY also has next-generation particles, consisting of organic and inorganic components, inside the columns. With a smaller diameter column, less sample is used. When linked to the new Waters Q-Tof Premier MS and Waters most recent bioinformatics approach, an LC/MS system that can target biomarker discovery (with the ability to do quantitative and qualitative protein profiling in just one pass) is the result. UltiMate uses a newly developed flow sensor for support of automated flow calibration and provides reproducible capillary and nano flow rates at 50 to 1 muL/minimum and greater than muL/minimum, respectively, and can be coupled to any MS. At Oak Ridge National Laboratory, scientists value the UltiMate systems for their flexibility in methodology and flow rates, says a senior research staff member at the Laboratory.